Figure 7
From: Structural organization and interactions of transmembrane domains in tetraspanin proteins
Cross-linking of single-cysteine mutants of CD9 protein. GFP fusions of CD9-TM(1+2) protein (panels A and B) or untagged full-length CD9 (panel C), and containing wild-type cysteines (Cys9,78,79), a single cysteine at positions indicated, or no cysteines (all replaced by Ser) were transiently expressed in human RD cells and cross-linked using CuP. Cell lysates were analyzed by Western blotting for GFP (panels A and B) or CD9 (panel C). % dimer is the fraction of dimer in total protein material (monomer + dimer), based on densitometry measurements of respective bands.