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Figure 6 | BMC Structural Biology

Figure 6

From: Deciphering structure and topology of conserved COG2042 orphan proteins

Figure 6

DTSSP cross-linked peptides identified by MALDI-TOF mass spectrometry. Protein samples were mixed with DTSSP for 30 min at room temperature at a molar ratio 1:20 (protein:DTSSP). They were then subjected to trypsin proteolysis. Masses of peptides treated with DTT (grey spectrum) or untreated (black spectrum) were measured by MALDI-TOF mass spectrometry. Peptide sequences are indicated and DTSSP cross-links or DTSSP moieties arising from DTT treatment are depicted schematically. Monoisotopic masses of protonated peptides [MH+] are theoretically: 1491.807 amu and 1493.822 amu for LVKLKIAEFTR [21-31] cross-linked with one DTSSP molecule (untreated (C68H115N16O17S2) and DTT-reduced (C68H117N16O17S2), respectively), 1835.846 amu and 1837.862 amu for VYIIDYHKDDPKR [3-15] cross-linked with one DTSSP molecule (untreated (C82H123N20O24S2) and DTT-reduced (C82H125N20O24S2), respectively).

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