Figure 1

First order pressure coefficients of the ¹H^N and of the ¹⁵N^H chemical shifts taken from the TROSY-HSQC spectra of hu PrP(23–230). Spectra were measured at 293 K at 600 MHz proton frequency. The absolute values of the conformation dependent pressure coefficients |B₁*(H)| (a) for the ¹H^N shifts and of the uncorrected pressure coefficient |B₁(N)| (b) for the ¹⁵N^H chemical shifts are plotted as function of the sequence position. The mean values of |B₁*(H)| and |B₁(N)| for the structured C-terminal and the unstructured N-terminal part of the protein are shown as solid lines, the mean values plus one standard deviation as dashed lines. The secondary structure is symbolized by arrows (β-strands) and lanyards (α-helices). P marks prolines, X other residues that are not visible or not assigned in the TROSY spectra.