Figure 5
From: Observation of intermediate states of the human prion protein by high pressure NMR spectroscopy
Pressure dependence of signal volumes in TROSY-HSQC spectra. The signal volumes are normalized to their volumes at ambient pressure and plotted as function of pressure. (a) Signal volumes from the N-terminus (residues 23–120) and (b) from the structured core (residues 121–230) are shown. Residues with no significant signal loss are highlighted green, disappearing signals are marked red. (c) Molar free energies at ambient pressure (ΔG0) for the N to I transition of hu PrP(121–230) calculated for individual residues assuming a slow exchange model. The mean value of ΔG0 (14.2 kJ/mol) is shown as solid line, the mean value plus/minus one standard deviation as dashed lines. Residues without a significant change in the signal volume are marked with #. O means, that the signal volume could not be determined due to heavy overlap of the peaks. P marks prolines, X other residues that are not visible or not assigned in the TROSY spectra.