Alignment of the Vc-HlyU sequence with those of templates SmtB from Synechococcus PCC7942, CzrA from Staphylococcus aureus, and CadC from Staphylococcus aureus pI258. Secondary structural elements are shown (α-helices as cylinders and β-strands as arrows) and labeled (α4 is the DNA-recognition helix, αR). Metal binding sites α3N and α5 are marked with plus (+) sign in magenta and green color, respectively. Residues which formed the homodimeric interface are shown in colors, core-residues and rim-residues  are shown in red and sky-blue, respectively. Conserved residues are denoted by asterix (*), while residues conserved among all the SmtB/ArsR family members are colored in blue asterix (*). The wild-type CadC has a Cys in position 11, which was Gly in the recombinant protein.