Figure 4

Genetic analysis of Asf1 residues important for histone binding. Asf1 histone binding mutants cause silencing defects in vivo. cac1Δasf1Δ cells were transformed with plasmids expressing the indicated Asf1 proteins. A four-fold dilution series was spotted onto rich media (YPD) to indicate cell number and onto media containing 5-FOA to measure silencing of a telomeric URA3 reporter gene.