Structural investigations of the ferredoxin and terminal oxygenase components of the biphenyl 2,3-dioxygenase from Sphingobium yanoikuyae B1

Table 3 Structurally analogous residues near the iron-sulfur cluster in various Rieske dioxygenase ferredoxins

BPDO-F_B1	BPDO-F_LB400	T4MOC-F	CARDO-F_CA10
Rieske cluster coordinating residues
CYS 45	CYS 43	CYS 45	CYS 46
HIS 47	HIS 45	HIS 47	HIS 48
CYS 64	CYS 63	CYS 64	CYS 65
HIS 67	HIS 66	HIS 67	HIS 68
Outer shell residues
ASN 43	ASP 41	ALA 43	ASP 44
GLY 48	GLY 46	GLN 48	GLY 49
ALA 50	TRP 48		ALA 51
PHE 66	LEU 65	ALA 66	PHE 67
GLY 69	GLY 68	TRP 49*	GLY 69
ALA 80	SER 79	ASN 80	SER 81
CYS 83	PRO 82		CYS 84

In the structural superposition, residues marked with an asterisk were more than 3.0 Å from the corresponding residue in BPDO-O_B1.

ISSN: 1472-6807