Figure 2

Apparent molar heat capacity curves measured by DSC for the R21A Spc-SH3 domain in the presence p41. The experiments were made in 20 mM glycine buffer, pH 3.0 at a protein concentration of 0.14 mM and variable concentrations of p41. Symbols represent the experimental curves for each p41/domain molar ratio (ranging from 0 to 9.8). The continuous lines correspond to the best global fitting using a model of coupled equilibria of binding and unfolding, as described in Supplementary material [Additional file 1].