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Table 1 Thermodynamic parameters of the binding between p41 and the WT and R21A variants of the Spc-SH3 domain at 25°C measured by ITC1

From: The high-resolution NMR structure of the R21A Spc-SH3:P41 complex: Understanding the determinants of binding affinity by comparison with Abl-SH3

pH

Protein

Kb (×10-3 M-1)

ΔHb (kJ mol-1)

p

TΔSb (kJ mol-1)

ΔGb (kJ mol-1)

7.0

R21A

18.1 ± 1.9

-58.7 ± 2.5

0.06 ± 0.05

-34.4

-24.3

 

WT

5.2 ± 1.2

-45.4 ± 6.3

0.01 ± 0.12

-24.2

-21.2

 

Abl-SH3

323

-91.6

0.1

-60.2

-31.4

3.0

R21A Spc-SH3

18.2 ± 2.0

-51.3 ± 1.7

-0.29 ± 0.08

-27.0

-24.3

 

WT Spc-SH3

5.8 ± 0.8

-43.9 ± 3.3

-0.07 ± 0.11

-22.4

-21.5

 

Abl-SH3

210

-93.3

-

-62.9

-30.4

  1. 1The similar parameters for the Abl-SH3 domain are also shown for comparison and are taken from reference 16. The errors in each parameter were estimated from 95% confidence intervals as determined from the fittings of the binding isotherms.