Additional rice MIPs in the phyologenetic tree of all rice MIPs. Phylogenetic analysis of all 39 rice MIP sequences is shown. This tree was created using the Neighbor-Joining method and the multiple sequence alignment for this purpose was generated by the T-Coffee program . As observed in Arabidopsis and maize, rice MIPs also can be classified into four subfamilies. OsPIPs, OsTIPs, OsNIPs and OsSIPs respectively indicate plasma membrane intrinsic proteins, tonoplast intrinsic proteins, Nod26-like intrinsic proteins and small basic intrinsic proteins from rice. Thirty three out of thirty nine sequences have been identified by Sakurai et al. . The additional six sequences identified in this study are shown within gray boxes.