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Table 3 Conservation of small and weakly polar residues at the helix-helix interfaces of plant MIP proteins

From: Homology modeling of major intrinsic proteins in rice, maize and Arabidopsis: comparative analysis of transmembrane helix association and aromatic/arginine selectivity filters

Residuea All families b, c PIPsc TIPsc NIPsc SIPsc
T48 T(93), A, S ( 97% ) T(100) ( 100% ) T(94) ( 94% ) T(92), S ( 96% ) T(63), A(25), S ( 100% )
T55 G(39), T(35), A ( 93% ) T(85), S ( 100% ) G(78), A ( 100% ) G(50), T, A ( 81% ) G(38), S(25), A ( 75% )
A78 A(77), S, C, T ( 93% ) A(95), S ( 98% ) A(88), S ( 100% ) A(62), C, S ( 96% ) T(38) ( 38% )
G82 G(63), A(28), S, T ( 94% ) G(100) ( 100% ) A(91), G, T ( 100% ) G(96) ( 96% ) S(38) ( 38% )
A103 A(88), S, T, C ( 96% ) A(100) ( 100% ) A(97) ( 97% ) A(85), S(15) ( 100% ) T(50), C ( 62% )
G107 G(61), A(32), S, T ( 100% ) G(97), A ( 100% ) G(78), A, S ( 100% ) A(77), T, S, G ( 100% ) A(100) ( 100% )
G129 G(90), A ( 100% ) G(100) ( 100%) G(81), A ( 100% ) G(81), A ( 100% ) G(100) ( 100% )
A130 A(63), S(36), G ( 100% ) A(97), G ( 100% ) S(59), A(41) ( 100% ) S(65), A(35) ( 100% ) A(75), S(25) ( 100% )
G133 A(55), G(44) ( 99% ) G(97) ( 97% ) A(97), G ( 100% ) A(100) ( 100% ) G(88), A ( 100% )
T172 T(91), S, A ( 100% ) T(100) ( 100% ) T(97), A ( 100% ) T(73), S(27) ( 100% ) T(75), S(25) ( 100% )
S181 A(40), S(40), G, T ( 93% ) S(100) ( 100% ) A(84), S, T ( 94% ) A(58), G(35), S, T ( 100% ) T(25), G ( 38% )
G203 G(91), A, S ( 99% ) G(100) ( 100% ) G(100) ( 100% ) G(92), A ( 96% ) S(75), A(25) ( 100% )
S226 S(71), T, A ( 97% ) S(97), T ( 100% ) S(75), A ( 94% ) T(54), S(46) ( 100% ) A(88) ( 88% )
G248 G(78), A, S, C ( 100% ) G(100) ( 100% ) G(100) ( 100% ) A(50), G(38), S ( 100% ) C(38), A(25), S(25), G ( 100% )
G252 G(97), A ( 100% ) G(100) ( 100% ) G(100) ( 100% ) G(100) ( 100% ) G(62), A(38) ( 100% )
A253 A(66), G, T, S ( 97% ) A(97), G ( 100% ) G(72), A(25), S ( 100% ) A(69), T ( 88% ) A(62), T(38) ( 100% )
A256 A(71), G(27) ( 98% ) A(97) ( 97% ) A(100) ( 100% ) G(100) ( 100% ) A(62), G(25), S ( 100% )
  1. a Residue numbers correspond to that of the plant aquaporin SoPIP2;1 (PDB ID: 1Z98).
  2. b Conservation is reported for all MIP subfamilies from the structure-based sequence alignments of 105 MIP sequences from rice, maize and Arabidopsis.
  3. c Small and weakly polar interfacial residues in plant MIP sequences and their conservation (if it exceeds 25%) are given. If the conservation is less than 25%, only the residues are reported. Group conservation of small and weakly polar residues (Gly, Ala, Ser, Thr and Cys) is shown in bold and italics.