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Table 2 Assignment of the sequence of luffaculin 1 by X-ray sequencing method (total number of residues: 241)

From: X-ray sequence and crystal structure of luffaculin 1, a novel type 1 ribosome-inactivating protein

  Sequence assignment method Number of identified residues
1 Both molecule A and B have clear electron density and can be assigned unambiguously 151
2 Using method 1 plus electron density of the second molecule for those residues where the first molecule has weak electron density, 66% of residues (160) can be deduced 160 (9 additional residues were assigned: A123, A185, A206, A215, A219, A221, A222, A234 and B209)
3 Using methods 1 and 2 plus chemical environment evaluation without using sequence comparison, 86% of residues (208) can be deduced. 208 (exclude 28*, 29, 33*, 48*, 56*, 93*, 97, 109*, 111*, 122*, 132*, 142*, 156*, 157*, 169*, 177*,179, 190*, 197*, 202*, 204*, 205*, 213*, 216*, 217, 218, 220*, 223*, 225, 226*, 228, 237*, 241*)
4 Using method 3 plus the sequence comparison, 94% of residues (227) can be deduced. 227 (exclude 28*, 29, 97, 109**, 111**, 190*, 216*, 217, 218, 220*, 225, 228, 223*, 237*)
  Neither molecule A nor B has clear density map, but the sequence is highly conserved among RIPs 4 (A&B109*, A&B179, A&B111*, A&B223*)
  Neither molecule A nor B has clear density map, and the sequence is not conserved among RIPs 6 (A&B29, A&B97, A&B217, A&B218, A&B225, A&B228)
  1. *These residues belong to residue pairs (Asn/Asp, Gln/Glu, Val/Thr) and cannot be distinguished by X-ray crystallography.
  2. **These two residues are conserved, but the electron density of both molecules A and B isn't clear, so we cannot classify these two residues to Group 4.