The pathway followed by the for LAO-binding Protein. The left most and right most structures are the unbound and bound conformations, respectively. The intermediate structures are obtained by adding the second global eigenvector to the original coordinates. Since the eigenvectors are normalized to one, a scaling factor of 40, 50 and 60 are used to obtain the intermediate structures. The rmsd values of the intermediate structures are 2.68 (2.55), 2.33 (3.18) and 2.13 (3.82) from the bound (unbound) structure. The two domains of the proteins close as the ligand is bound to the protein.