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Figure 8 | BMC Structural Biology

Figure 8

From: Structure of the dimeric N-glycosylated form of fungal β-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies

Figure 8

Effect of glycosylation on enzymatic activity and stability of β- N -acetylhexosaminidase. (A) deglycosylation of the enzyme by endoglycosidase H and N-glycanase under native conditions. β-N-acetylhexosaminidase (0.1 μg, lane 1) was deglycosylated using 10 U (lane 2), 5 U (lane 3), 2 U (lane 4), 1 U (lane 5), 0.5 U (lane 6), 0.2 U (lane 7), 0.1 U (lane 8), or 0.05 U (lane 9) of endoglycosidase H (Endo Hf). Lane 10 contains Endo Hf control, and lane 11 β-N-acetylhexosaminidase (0.1 μg) deglycosylated by 0.1 U of N-glycanase. (B) comparison of the enzymatic parameters of native and deglycsylated β-N-acetylhexosaminidase. (C) effect of β-N-acetylhexosaminidase deglycosylation on the stability of the enzyme under various pH values. The average values from triplicate determinations with the standard error indicated by the error bars are shown.

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BMC Structural Biology

ISSN: 1472-6807

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