Structure of the dimeric N-glycosylated form of fungal β-N-acetylhexosaminidase revealed by computer modeling, vibrational spectroscopy, and biochemical studies

Table 2 Secondary structure estimation (in %) of β-N-acetylhexosaminidase by FTIR and Raman spectroscopy compared with the model structures

Method	Secondary structure estimation
	α-Helix	β-Sheet	β-Turn	Bend	Other
Model	32	15	14	14	25
FTIR-LSA^a	31	19	14	14	22
Raman-LSA^b	31	26	15	--	26

^a Least-squares analysis of FTIR amide I and II bands according to [55]. ^b Least-squares analysis of Raman amide I band according to [54].

ISSN: 1472-6807