Figure 4

(A) A graphic display of the immunity activity of L. sakei strains possessing PedB and PedB variants to pediocin PP-1. The results are presented using the same methods as in Figure 1c. The results represent the averaged data from at least three experiments, and the standard deviation values are indicated in each bar. (B) Far-UV CD spectra of PedB and C-terminal shortened PedB variants. CD spectra (190~250 nm) of 25 μg/ml ml wild type PedB and PedB variants were obtained to compare the secondary structure at 25°C. (C) Near-UV CD spectra of PedB and C-terminal shortened PedB variants. CD spectra (240~360 nm) of 200 μg/ml wild type PedB and PedB variants were obtained to compare the tertiary structure and protein folding at 25°C.