Figure 2

Purification of mutants by affinity chromatography. Comparison of chromatograms from purification of PA-IIL mutants S22A (black), S23A (blue), G24N (green) and PA-IIL wild type (red) by affinity chromatography on Mannose agarose column (HR 10/10). Loading buffer: 20 mM Tris/HCl, 100 mM NaCl, 100 μM CaCl₂, pH 7.5; Elution buffer: 20 mM Tris/HCl, 100 mM NaCl, 100 μM CaCl₂, 0.1 M D-mannose, pH 7.5; Sample: cytoplasmic soluble protein fractions.