Comparison of S22A/RSIIL and G24N/PA-IIL with Me-α-Man with important linkages highlighted. A) PA-IIL, B) G24N, C) RS-IIL, D) S22A in a surface/stick representation. Mutated amino acids are in light brown. Interactions responsible for sugar preference are in yellow, newly created hydrogen bonds are in blue. Figure clearly demonstrates different orientation of O6 of methyl mannoside.