Table 3 Thermodynamics of lectin/sugar binding. Thermodynamics of binding for different monosaccharides interacting with PA-IIL and its mutants, compared to thermodynamic characteristics of mannose-preferring lectin RS-IIL from Ralstonia solanacearum as determined by ITC at 25°C. Standard deviations calculated from three independent measurements for all ligands.

Me-α-L-Fuc

G24N

524 (± 36.7)

0.19 (± 0.03)

-38.4 (± 0.2)

-40.1 (± 0.7)

1.8 (± 0.7)

S23A

418 (± 72.8)

0.25 (± 0.01)

-37.8 (± 0.4)

-33.9 (± 1.2)

-3.8 (± 0.9)

PA-IIL ^a

235 (± 8.0)

0.43 (± 0.01)

-36.4 (± 0.1)

-41.3 (± 1.0)

4.9 (± 1.0)

S22A

26.4 (± 4.9)

3.96 (± 0.91)

-30.9 (± 0.5)

-37.1 (± 0.6)

6.2 (± 0.9)

RS-IIL^b

< 1

> 100

L-Fuc

S23A

23.8 (± 3.4)

4.3 (± 0.6)

-30.6 (± 0.4)

-26.9 (± 0.2)

-3.7 (± 0.6)

G24N

15.2 (± 2.3)

6.6 (± 0.9)

-30.0 (± 0.4)

-31.1 (± 0.4)

1.1 (± 0.7)

PA-IIL ^c

12.0 (± 1.0)

8.3 (± 0.7)

-29.0 (± 0.1)

-27.7 (± 0.5)

-1.3 (± 0.5)

S22A

10.3 (± 0.4)

9.7 (± 0.4)

-28.6 (± 0.1)

-26.8 (± 0.7)

-1.8 (± 0.6)

RS-IIL

nd

nd

nd

nd

nd

Me-α-L-Gal

PA-IIL ^a

85.0 (± 5.0)

1.17 (± 0.07)

-33.8 (± 0.1)

-31.5 (± 0.6)

-2.3 (± 0.7)

G24N

82.2 (± 18.8)

1.29 (± 0.35)

-33.7 (± 0.6)

-18.7 (± 0.3)

-15.0 (± 0.9)

S23A

81.6 (± 14.4)

1.23 (± 0.07)

-33.9 (± 0.4)

-26.8 (± 1.7)

7.1 (± 1.3)

S22A

6.75 (± 0.31)

15.0 (± 0.66)

-27.6 (± 0.5)

-19.2 (± 0.2)

-8.3 (± 0.3)

RS-IIL

nd

nd

nd

nd

nd

Me-β-D-Man

RS-IIL

438 (± 2.0)

0.23 (± 0.01)

-37.9 (± 0.1)

-24.9 (± 0.2)

-13.0 (± 0.2)

S22A

36.0 (± 1.8)

2.78 (± 0.14)

-31.7 (± 0.1)

-24.3 (± 0.6)

-7.4 (± 0.7)

G24N

2.34 (± 0.17)

42.9 (± 3.12)

-24.9 (± 0.2)

-27.1 (± 0.8)

2.1 (± 0.9)

S23A

1.91 (± 0.33)

54.0 (± 9.32)

-24.4 (± 0.4)

-16.7 (± 2.3)

-7.7 (± 2.7)

PA-IIL ^a

1.42 (± 0.05)

71.0 (± 3.00)

-23.7 (± 0.1)

-17.8 (± 1.3)

-5.9 (± 0.3)