Figure 2
Cations influence enzymatic activity of both MtαIPMS and the TIM barrel domain. Effect of KCl, MgCl2, ZnCl2 or CdCl2 on enzymatic activity of MtαIPMS (Panel A) and the catalytic TIM barrel Domain (Panel B). In both the panels the bars 1 to 4 represent samples of protein in presence of 20 mM KCl, 20 mM KCl + 5 mM MgCl2, 20 mM KCl + 5 mM MgCl2 +2 mM ZnCl2 and 20 mM KCl + 5 mM MgCl2 + 2 mM CdCl2, respectively. The data is represented as percent activity with the activity of the protein in presence of 20 mM KCl taken as 100%. The data is represented as mean ± SD of three values.