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Figure 7 | BMC Structural Biology

Figure 7

From: Cation induced differential effect on structural and functional properties of Mycobacterium tuberculosis α-Isopropylmalate synthase

Figure 7

Structural and functional properties of MtαIPMS and the TIM barrel domain. A. Far-UV CD spectra of MtαIPMS (profile 1) and the TIM barrel domain (profile 2). B. Tryptophan emission spectra of MtαIPMS (profile 1) and the TIM barrel domain (profile 2). C. Thermal unfolding of MtαIPMS and the TIM barrel domain. Effect of increasing temperature on the CD ellipticity at 222 nm of MtαIPMS (profile 1) and the TIM barrel domain (profile 2), respectively. The values have been represented as percentage with the value obtained at 25°C for each sample taken as 100 percent, respectively. D. Enzymatic activity of MtαIPMS and the TIM barrel domain. Relative enzymatic activity of MtαIPMS (Bar 1 and 2) and the TIM barrel domain (Bar 3 and 4) at 25 and 65°C, respectively. The values have been represented as percent activity with the value observed for MtαIPMS at 25°C taken as 100%. The data is represented as mean ± SD of three values. Inset shows the loss of enzymatic activity of the MtαIPMS (closed symbols) and the TIM barrel domain (open symbols) on incubation with increasing concentration of L-lecucine. The data has been represented as percentage with the activity of MtαIPMS or the TIM barrel domain in absence of Leucine taken as 100 percent. E. SDS-PAGE profile of glutaraldehyde cross-linked TIM barrel domain. Lanes 1–3 represent molecular weight markers, uncrosslinked and glutaraldehyde cross-linked TIM barrel domain, respectively.

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