Figure 1
DSC transition curves of muscle fibres in different intermediate states of the ATP hydrolysis (Rigor (dashed line), ADP.Vi (solid line), AMP.PNP (dotted line), ADP.BeFx (dash-dot line)). The melting temperature of the last transition depends strongly on the ligand occupied by the nucleotide binding sites of myosin and/or actin. The heat flow measured in mW is plotted against temperature after baseline correction. The middle transition is not affected by the nucleotides and nucleotide analogues.