Figure 5

DSC transitions of muscle fibres in the presence of ADP and BF_x (solid line). DSC traces of myosin rod and the actin population at about 65°C were subtracted (dotted line), using the single Gaussian contributions from the deconvolution. The residual curve shows the transitions of myosin domains and that actin population affected by the nucleotide analogue. Fibre bundle was reacted in a medium containing 4 mM ADP plus 4 mM BeFx in rigor buffer for 15 min before DSC measurement was taken.