Figure 5

Model for temperature-sensitive mutant (D ts 30) of vvUDG. This figure shows a close-up view of the protein region for the G179R substitution in the temperature-sensitive mutant Dts 30 and shows the effect of the mutation. (A) Residues in this pocket are displayed as stick models and are labeled. (B) The pocket is shown in the same view as in Fig. 5A, but an Arg residue (color code: pink) in one rotamer conformation was modeled in place of G179 to indicate that a substitution at this position will introduce steric hindrance. In addition, other rotamer conformations that point towards residues F195 and I198 will position this charged residue even farther into the hydrophobic pocket. In the shown conformation, distances of side chain atoms of the modeled R179 are only 1.5–2.5 Å from surrounding residues.