Figure 3

(NZ)CHN contacts and water molecules in (NZ)CHO bonds observed for the methylated nuclease. A, B. The side chain nitrogens of R35 and Q31 are seen forming (NZ)CHN bonds with the methyl groups of MLY172 and MLY159 respectively. Excess positive charge on the nitrogen is dispersed via ionic interactions mediated by water molecules [22] or as in case of Q31, relayed directly to electron negative oxygen OE1. C. A number of water molecules are seen participating in the formation of (NZ)CHO bonds. Residues involved in the formation of (NZ)CHN and (NZ)CH...O bonds are represented as sticks while water molecules are represented as spheres.