Protein | [GdmCl]1/2 |
m
| ΔGU,H2O |
|---|
| | (M) | (kJ/mol/M) | (kJ/mol) |
Nuclease | 3.01 ± 0.01 | 19 ± 1 | 57.4 ± 3.0 |
Nuclease (modified) | 2.74 ± 0.01 | 14.4 ± 0.5 | 39.4 ± 1.4 |
- § The values for GdmCl denaturation were obtained by fitting data to a standard 2-state model [29] where [GdmCl]1/2 is the midpoint and m the slope of the denaturation transition. ΔGU,H2O is the free energy of unfolding at 25°C in the absence of denaturant. The errors shown are the standard errors from the fit. The dependence of ΔGU on the GdmCl concentration is given by ΔG(GdmCl) = Δ Gu-m [GdmCl]. Conditions were as described in the legend for Figure 5A. The thermal denaturation data (Figure 5B) was also fitted to a 2-state model and was extrapolation to standard conditions (25°C in the absence of denaturant) as described previously [30]. The value of ΔGU,H2O thus obtained is the same within the error as that shown above.
