Figure 4From: Type II restriction endonuclease R.Eco29kI is a member of the GIY-YIG nuclease superfamilyBinding of wt R.Eco29kI (a), H108F (b) and N154L (c) mutants to 24-mer DNA duplex containing the CCGCGG R.Eco29kI recognition site. The Kd values were determined by direct titration using the 24-mer DNA duplex as the fluorescent probe. Serial dilutions of wild type and mutant proteins were incubated with 1 nM FAM-labeled duplex.Back to article page