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Table 1 DNA cleavage and binding activities of wt R.Eco29kI and its mutants

From: Type II restriction endonuclease R.Eco29kI is a member of the GIY-YIG nuclease superfamily

R.Eco29kI DNA cleavage activity [% of wt] standard bimolecular binding model cooperative binding model
   Kd, nM χ2 R2 Kd, nMn n χ2 R2
WT 100 16 ± 5 1500 0.78 299 ± 50 2.1 ± 0.2 360 0.95
Y49A 0 49 ± 5 105 0.97 529 ± 50 1.9 ± 0.3 31 0.99
R86A 100 21 ± 5 1232 0,82 344 ± 19 2.0 ± 0.4 415 0.95
R104A ~2 43 ± 18 442 0.94 1911 ± 356 1.8 ± 0.2 27 0.99
H108F 0 114 ± 25 1533 0.84 14285 ± 2000 2.0 ± 0.1 582 0.94
E142A 0 45 ± 11 1480 0.87 970 ± 200 1.8 ± 0.4 547 0.95
N154L ~10 50 ± 15 1267 0.75 3100 ± 700 2.0 ± 0.3 431 0.91