Table 1 DNA cleavage and binding activities of wt R.Eco29kI and its mutants
From: Type II restriction endonuclease R.Eco29kI is a member of the GIY-YIG nuclease superfamily
R.Eco29kI | DNA cleavage activity [% of wt] | standard bimolecular binding model | cooperative binding model | |||||
|---|---|---|---|---|---|---|---|---|
|  |  | Kd, nM | χ2 | R2 | Kd, nMn | n | χ2 | R2 |
WT | 100 | 16 ± 5 | 1500 | 0.78 | 299 ± 50 | 2.1 ± 0.2 | 360 | 0.95 |
Y49A | 0 | 49 ± 5 | 105 | 0.97 | 529 ± 50 | 1.9 ± 0.3 | 31 | 0.99 |
R86A | 100 | 21 ± 5 | 1232 | 0,82 | 344 ± 19 | 2.0 ± 0.4 | 415 | 0.95 |
R104A | ~2 | 43 ± 18 | 442 | 0.94 | 1911 ± 356 | 1.8 ± 0.2 | 27 | 0.99 |
H108F | 0 | 114 ± 25 | 1533 | 0.84 | 14285 ± 2000 | 2.0 ± 0.1 | 582 | 0.94 |
E142A | 0 | 45 ± 11 | 1480 | 0.87 | 970 ± 200 | 1.8 ± 0.4 | 547 | 0.95 |
N154L | ~10 | 50 ± 15 | 1267 | 0.75 | 3100 ± 700 | 2.0 ± 0.3 | 431 | 0.91 |