Figure 1

Sequence comparison of M. tuberculosis IMPase-like proteins with human IMPase. Sequences were aligned based on the structural superimposition of SuhB and human IMPase using STRAP [53], and formatted using ESPript [54]. Secondary structure elements of SuhB are above the sequence, cylinders representing helices and arrows β-strands, coloured according to Fig. 2A. Full asterisks denote residues coordinating the catalytic Mg²⁺ sites, triangles indicate residues contacting inositol-1-phosphate in the simulated SuhB-substrate complex. Horizontal boxes indicate disordered parts of the sequence. The grey arrows indicate the positions of strands β1, β2 in the α1-α2 loop ('mobile loop') of human IMPase, with the open asterisk marking the critical lysine residue.