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Figure 5 | BMC Structural Biology

Figure 5

From: Dimerization of inositol monophosphatase Mycobacterium tuberculosis SuhB is not constitutive, but induced by binding of the activator Mg2+

Figure 5

Analytical ultracentrifugation in sedimentation velocity mode demonstrates that SuhB is monomeric in the absence of Mg2+. Main panel: Traces of the molecular weight distribution of M. tuberculosis SuhB in a sedimentation velocity experiment. Conditions analysed were 0.5 mg.ml-1 SuhB (solid triangle), 1.0 mg.ml-1 SuhB (open triangle),1.0 mg.ml-1 SuhB + 1 mM Mg2+ (open circle). The main peak is centred at 30,508 Da, the secondary peak at 54,160 Da. Inset: enlarged view of the region between 40,000 and 80,000 Da. Samples were centrifuged at 40,000 rpm, 4°C, for a minimum of 12 hours, in 20 mM Tris-HCl pH 7.9, 50 mM NaCl and MgCl2 as indicated.

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