Dimerization of inositol monophosphatase Mycobacterium tuberculosis SuhB is not constitutive, but induced by binding of the activator Mg2+

Table 3 BLAST search for M. tuberculosis IMPase homologues of known structure

	SuhB	Rv3137	ImpA	CysQ	1IMA^a	2BJI	1JP4	2P3N	1G0H
SuhB	100	30	23	22	25	25	14	23	19
Rv3137		100	23	18	23	22	22	29	17
ImpA			100	13	15	16	12	22	20
CysQ				100	14	12	16	7	3
1IMA (IMP human)	2.0E-20				100	88	16	28	19
	(194)
2BJI (IMP bovine)	3.0E-21	4.7E-07				100	19	28	20
	(194)	(165)
1JP4 (PIPase_Rno)				2.7E-05			100	18	15
				(169)
2P3N(TM1415_Tma)		9.5E-16	4.0E-12	8.7E-04				100	23
		(221)	(247)	(192)
1G0H (MJ0109_Mja)			6.3E-10						100
			(161)

Fields above the table diagonal give the %-identity in a STRAP-generated multiple sequence alignment. Fields in bold type give the E-values and (number of paired amino acid residues) of the two top hits in the BLAST search of the PDB [48].
^a References for PDB entries:
1IMA – human IMPase complexed with myo- inositol-1-phosphate [29]
2BJI – bovine IMPase [17]
1JP4 – Rattus norvegicus 3'-phosphoadenosine 5'-phosphate and inositol-1,4-bisphosphate phosphatase [22]
2P3N – Thermotoga maritima TM1415 IMPase/FBPase [27]
1G0H – Methanococcus jannaschii MJ0109 IMPase/FBPase [24].

ISSN: 1472-6807