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Figure 5 | BMC Structural Biology

Figure 5

From: Grb7 SH2 domain structure and interactions with a cyclic peptide inhibitor of cancer cell migration and proliferation

Figure 5

Isothermal titration calorimetric measurement of G7-18NATE peptide binding to the Grb7 SH2 domain (a) The sequence of the G7-18NATE peptide used in this study. (b) ITC data showing the titration of G7-18NATE into Grb7 SH2. The samples were prepared in 50 mM MES pH 6.6, 100 mM NaCl and 1 mM DTT. ITC experiments were carried out in duplicate at 25°C with Grb7 SH2 in the cell at 25 μM and G7-18NATE in the syringe at 440 μM. The upper panel shows the raw data while the lower panel shows the data after peak integration and subtraction of the heat-of-dilution control. In this panel the grey line represents the fit to a 1:1 binding model.

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BMC Structural Biology

ISSN: 1472-6807

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