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Table 3 Double mutant cycle of BMP-2 and ActR-IIBECD (Biosensor analysis)

From: A silent H-bond can be mutationally activated for high-affinity interaction of BMP-2 and activin type IIB receptor

 

Receptor ectodomain proteins

BMP-2a (immobilized)

ActR-IIB

ActR-IIBL61A

ActR-IIBL61P

 

app. KD in Nm

   wildtype

2700

42000

76000

L100K/N102D

140

14000

160000

   Act-Ab

60

2000

180000

  1. a) BMP-2 variants were expressed in E. coli.
  2. b) the apparent binding constant of Act-A is shown for comparison.