Right angle light scattering timedrive plots for BRD2, BioBRD2 and mixtures of the two peptides in the presence of lanthanum. The proteins were added at 100 (BRD2) and 200 s (BioBRD2) and the metals were introduced using a syringe pump starting at 500 s for a total of 120 s. A solution of EDTA was added to the cuvette using a syringe pump beginning at 1200 s until the right angle light scattering signal fell to its original level. A) Timedrive illustrating the relative right angle light scattering responses of 10 μM BRD2 (blue line) 10 μM (yellow line), 20 μM (purple line), 30 μM (green line) and 60 μM (red line) BioBRD2. It is clear that BioBRD2 does not produce increases in right angle light scattering comparable to BRD2, suggesting it does not form stable filaments. B) Timedrive illustrating the relative right angle light scattering responses of 10 μM BRD2 (blue line) BioBRD2 alone (yellow line), a 1 to 1 (cyan line), 1.5 to 1 (purple line), 4 to 1 (green line) and 9 to 1 (red line) excess of unmodified BRD2.