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Table 1 Effects of various mutations on the activation of the sweet taste receptor by neohesperidin dihydrochalcone (NHDC) and aspartame (ASP)

From: The binding site for neohesperidin dihydrochalcone at the human sweet taste receptor

variant NHDC EC50 (mM) NHDC maximal signal (% of wt) ASP EC50 (mM) ASP maximal signal (% of wt)
wt 0.1 ± 0.06 100 1.3 ± 0.1 100
Q636A3.28 0.3 ± 0.2 61 ± 29 1.1 ± 0.1 70 ± 18
Q637E 3.29 1.5 ± 0.4 51 ± 13 1.7 ± 0.2 51 ± 10
S640A 3.32 0.8 ± 0.2 63 ± 18 1.4 ± 0.5 61 ± 40
H641A 3.33 1.2 ± 0.4 57 ± 22 1.8 ± 0.4 79 ± 70
H721A ex2 0.8 ± 0.3 66 ± 10 1.6 ± 0.3 60 ± 40
R723A5.36 0.2 ± 0.1 69 ± 71 1.5 ± 0.5 68 ± 50
S729A5.42 0.2 ± 0.1 80 ± 16 0.9 ± 0.4 72 ± 20
F730L 5.43 0.9 ± 0.1 70 ± 18 1.7 ± 0.4 87 ± 27
A733V5.46 0.1 ± 0.02 133 ± 8 1.1 ± 0.3 92 ± 80
H734A5.47 n.f. -- n.f. --
W775A 6.48 >3 30 ± 41 1.9 ± 0.2 20 ± 30
F778A 6.51 1.5 ± 0.4 50 ± 18 1.2 ± 0.1 80 ± 80
V779A6.52 0.2 ± 0.1 88 ± 14 1.2 ± 0.3 52 ± 10
L782A 6.55 >3 50 ± 11 1.7 ± 0.3 95 ± 90
R790Qex3 0.1 ± 0.06 77 ± 15 0.9 ± 0.4 71 ± 30
L798I7.36 0.2 ± 0.2 66 ± 26 0.9 ± 0.4 81 ± 5
  1. Receptor variants that affect the NHDC response are shown in bold. Values are given as average over at least three independent experiments ± SD. n.f., no response to any tested sweetener. (>), EC50 not calculable because saturation is not reached (Additional file 2).