Skip to main content

Table 1 Primers used in this study

From: The structure of Mycobacteria 2C-methyl-D-erythritol-2,4-cyclodiphosphate synthase, an essential enzyme, provides a platform for drug discovery

Primer

5' Sequence*

IspFNFor

AAGCTTgtacgagttcccgctgaaaacgc

IspFNRev

GGATCCgagagtctgcccgtcgagctg

IspFCFor

GGATCCgcaatcgctacggcattggtggt

IspFCRev

GGTACCaccaccaccgacgggctgggc)

IspDFSh

TTAATTAAgacgccaaagccgagaccatcctt

IspDFRev

TTAATTAAgccagcttacctgcccaattgctg

IspFIntA

ggtcgaatcgcactgacac

IspFIntB

cgatcatctgggtgatatgc

MsIspFNter

CATATGatgttgcctcgcgtagggc

MsIspFCter

GGATCCtactttgcatcaccgaccggtt

IspDUSFor

gacgagaatcaatgagacct

IspDUSRev

agtgatatcggctcggtgac

  1. *Upper case letters indicate inserted restriction sites.