Figure 3

LepFNR SAXS data. (a) Experimental solution scattering curve of LepFNR and theoretical scattering intensities. Desmeared experimental curve is shown as dots with error bars; theoretical scattering intensity from the DAM is given in continuous line; scattering intensity from the LepFNR monomer is shown as a broken line. An inset displays the correspondent Guinier plot. (b) Comparison of distance distribution functions for LepFNR. The p(r) curves obtained were calculated using the GNOM program, the experimentally derived distribution for LepFNR is shown as dots with error bars. Distributions were calculates for the DAM (continuous line) and a single monomer of LepFNR crystal structure (broken line). (c) Stereoviews showing the superposition of the high-resolution crystallographic monomer of LepFNR with the envelope obtained from the data SAXS (spheres). Middle and bottom images are rotated by 90° around the y and x axes, respectively, compared to the top image.