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Table 3 Kinetic and binding parameters for LepFNR NADPH-ferricyanide diaphorase activity

From: Crystal structures of Leptospira interrogans FAD-containing ferredoxin-NADP+ reductase and its complex with NADP+

FNR

Kd (μM, NADP+)

Km (μM, NADPH)

kcat (s-1)

kcat/Km (μM-1·s-1)

L. interrogans

43 ± 3

19.5 ± 1.7

258 ± 13

13.2

P. sativum

32 ± 2

19.0 ± 1.6

324 ± 16

17.1

  1. The different parameters were obtained as described in Experimental Procedures. Each parameter value represents the average of 3 independent experiments.
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BMC Structural Biology

ISSN: 1472-6807

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