Figure 1

Detergent extraction screen. (A) NuPAGE (4–12%) Bis-Tris SDS gel of the high-speed spin (125,000 g, 2 h) supernatant of the membrane fraction of QseC, extracted overnight by different detergents. Each well was loaded with 10 μ l of a 1:1 supernatant-to-loading buffer solution, except for the SDS sample, for which 2 μ l was loaded to adjust for a comparable amount of protein in the gel. (B) The detergent retention ratio defined as fc/ic is shown for each detergent of panel A (ic is the initial detergent concentration, fc is the final detergent concentration, both determined by NMR).