Figure 2
From: NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes
Detergent concentration screen. (A) NuPAGE (4–12%) Bis-Tris SDS gel of the high speed spin (125,000 g, 2 h) supernatant from the overnight extraction of the membrane fraction. Extraction detergents FC14, Zw-3.14, DPh, and SDS with increasing concentrations were tested on QseC and KvPae. Sample loading is as in 1A. (B) The detergent retention ratios for each extraction condition.