Figure 4

Dependence of the detergent concentration factor on the initial detergent concentration. Dependence of the concentration factor (see Methods) on the initial LDAO concentration. Detergent samples were prepared in 20 mM Tris-HCl buffer, pH 8.0 and concentrated 10× using Vivaspin 30 KDa cutoff ultrafiltration devices. Concentration factors were calculated using concentration measurements based on three different peaks in ¹H-NMR spectra (at 3.11, 1.27 and 0.86 ppm). For the initial concentrations of 2.8 and 6.8 mM, three different devices were used and the concentration factors were calculated for each sample (additional data points are shown as "+"). The least-squares regression line and the corresponding R² are shown.