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Table 2 Characteristic 1H signals of used detergents.

From: NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes

Detergent

Group

Chemical shift [ppm]

Number of protons

Minimal detectable concentration [×10-3 mM]a

FC14, FC12

(CH3)3-N-

3.23

9

10

 

CH3-

0.86

3

30

DDM, DM

-C1H-

4.45

1

200b

 

-C1H-

5.34

1

200b

 

CH3-

0.86

3

30

DPh

CH3-

0.86

3

30

 

-N-((CH2)2-COO-)2

2.4–3.1c

8

30

SDS

CH3-

0.86

3

30

 

-CH2-(SO4)-

4.02

2

50

NG, OG

-C1H-

4.45

1

200b

 

CH3-

0.86

3

30

Zw-3.14, LDAO

-(CH3)2

3.11

6

15

 

CH3-

0.86

3

30

DSS (reference)

(CH3)3-Si-

0.00

9

 
  1. a – approximate lower limit of measurable concentration for the samples in 10–20% D2O, measured using Varian NMR System 500 MHz, probe sensitivity 1:350, 64 scans.
  2. b – 100 × 10-3 mM in 100% D2O.
  3. c – the chemical shifts of these signals are pH- and concentration-dependent.
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BMC Structural Biology

ISSN: 1472-6807

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