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Table 3 Detergent concentration factors cf (see Methods) for the Amicon and Vivaspin concentrators with 30 and 50 KDa cutoffs for several detergents, using initial concentrations ic, and fold concentrations set as 4× for Amicon and 5× for Vivaspin, unless marked otherwise.

From: NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes

  

Amicon

Vivaspin

   

cf

 

cf

Detergent

MW micelle [kDa]

ic [mM]

30 kDa

50 kDa

ic [mM]

30 kDa

50 kDa

FC14

46 a

0.34 b

0.48

0.30

0.34 b

0.39

0.03

  

0.55

0.79

0.73

0.43

0.48

0.03

DDM

40–76 c, d

0.35 b

0.31

0.23

0.35 b

0.17

0.00

  

0.54

0.64

0.56

0.54

0.57

0.00

Zw-3.14

30–47 e

1.04

0.62

0.40

1.17

0.31

0.21

FC12

18–21 c

3.60 b

0.30

0.21

3.60 b

0.05

0.01

  

9.20 b

0.52

0.23

9.20 b

0.24

0.02

DM

33.3 c

7.22

0.55

0.46

8.33

0.62

0.01

SDS

18–29 e

9.21

0.33

0.28

10.00

0.71

0.31

DPh

20–30 f

9.28

0.31

0.29

9.87

0.42

0.07

LDAO

17 g

0.51 b

0.01

0.01

0.51 b

0.00

0.00

  

2.18 b

0.32

0.27

2.18 b

0.15

0.01

  

5.10 b

0.50

0.32

5.10 b

0.28

0.04

  

10.28

0.68

0.64

10.28

0.75

0.20

NG

15–30 f

16.50

0.13

0.01

18.93

0.39

0.03

OG

8–26 e, h

38.30

0.05

0.00

44.17

0.21

0.03

  1. a – [8]; b – the sample was concentrated 10×; c – [24]; d – [28]; e – [25]; f – our estimates; g – [27]; h – [32].