Table 4 Estimated oligomeric state of EnvZ in DDM exchanged from both FC14 and DPh, EnvZ in FC14, Etk in DDM exchanged from FC14, and Etk-NM in FC12. The solution of Tanford's equation for EnvZ in DDM, which satisfied the physical constraints (see Step 4), is highlighted.
From: NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes
| Â | Â | Detergent | Â | Â | Â | |
|---|---|---|---|---|---|---|
Protein | M pm [kDa] | Extraction | Exchange | Fitted mass [kDa] | n | M d /M p |
EnvZ | 65.206 | FC14 | FC14 | Aggregates | Â | Â |
| Â | Â | Â | DDM | Aggregates | Â | Â |
| Â | Â | DPh | DDM | 112.2 | 1 | 0.93 |
| Â | Â | Â | Â | Â | 2 | -0.18 |
Etk | 99.788 | FC14 | DDM | ~70% aggregates | Â | Â |
| Â | Â | Â | FC12 | ~40% aggregates | Â | Â |
Etk-NM | 84.737 | FC12 | FC12 | 83.3 | 1 | Â |
| Â | Â | Â | FC12* | 80.2 | 1 | Â |