Conformational properties of latexin. A) CD spectrum of latexin at increasing urea concentrations. B) Overlay of the urea denaturation curve monitored by intrinsic fluorescence and by far UV-CD. The difference in the two transition midpoints is usually associated with the presence of an intermediate state whose population is theoretically calculated from the normalized unfolding curves. C) 3D structure of latexin (2BO9). The five triptophans (red) are located in the C-terminal subdomain (light blue and green) and the region corresponding to the main hot spot is located in the alpha-helix 3 (green).