Figure 2From: NMR and X-ray analysis of structural additivity in metal binding site-swapped hybrids of rubredoxinCorrelations among all hybrid and parental rubredoxin 1H chemical shifts. The amide chemical shift data of Figure 1 were used to assign each individual residue of the metal binding site-swapped hybrids to a particular parent. Panel A illustrates the comparison to parental chemical shifts for the metal binding site-swapped Cp rubredoxin, while the analogous 1H data for the complementary metal binding site-swapped Pf A2K rubredoxin hybrid are shown in panel B. Only the Hε resonances of Lys 46 (open diamonds) deviate substantially from equivalence.Back to article page