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Table 1 Differential chemical shifts between pairs of hybrid, Cp and Pf A2K rubredoxins.

From: NMR and X-ray analysis of structural additivity in metal binding site-swapped hybrids of rubredoxin

 

1HN

15N

1H(all)

1HN(con)a

15N(con)

1H(con)

Pf metal site-swapped Cp hybrid-parentb

0.046

0.279

0.042

0.049

0.318

0.043

Pf metal site-swapped Cp hybrid-non-parentc

-

-

-

0.301

1.872

0.191

Cp metal site-swapped Pf hybrid-parent

0.044

0.264

0.038

0.049

0.277

0.042

Cp metal site-swapped Pf hybrid-non-parent

-

-

-

0.334

1.871

0.175

Cp - Pf A2K

-

-

-

0.286

1.785

0.181

  1. a. Resonances from residues that are conserved between the parental sequences.
  2. b. Residue-specific parental type assignment based on minimal amide chemical shift difference.
  3. c. Cp or Pf A2K resonance not assigned to the hybrid resonance based on amide chemical shift.