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Table 1 FXa binding kinetic parameters and effect on prothrombinase activity of Viperidae SVPLA2

From: Characterization of a human coagulation factor Xa-binding site on Viperidae snake venom phospholipases A2 by affinity binding studies and molecular bioinformatics

PLA2 <kon>(M-1s-1) <koff> (s-1) <Kdapp>[nM] IC50[nM] pI*
CBc (3.2 ± 0.2) × 105 (1.6 ± 0.4) × 10-4 0.6 ± 0.3 0.7 ± 0.3 8.74
MtxII (10.4 ± 0.3) × 106 (1.78 ± 0.4) × 10-2 1.8 ± 0.9 3 ± 1 9.10
CbII (4.2 ± 2.5) × 105 (8.5 ± 2) × 10-3 20 ± 3 20 ± 4 8.96
AtxA (2.2 ± 0.3) × 105 (7 ± 1) × 10-3 30 ± 2 25 ± 5 8.35
CBa 2 (2.9 ± 0.4) × 105 (1.5 ± 0.2) × 10-2 52 ± 4 41 ± 5 8.74
VRV-PLVIII (4.5 ± 0.4) × 104 (2.6 ± 0.4) × 10-2 578 ± 15 130 ± 20 8.35
b Ahp (4 ± 1.5) × 104 (1.6 ± 0.3) × 10-2 400 ± 20 90 ± 10 8.71
Vbb (3 ± 0.5) × 104 (2.5 ± 0.4) × 10-2 830 ± 15 90 ± 30 8.64
AGTX NB NB NB >10 000 5.43
CA NB NB NB >10 000 -
Catx NB NB NB >10 000 4.64
CbI NB NB NB >10 000 4.86
Reduced and carboxymethylated CBc NB NB NB nd nd
hsPLA 2 (2.0 ± 0.8) × 106 (2.9 ± 1) × 10-2 14 ± 2 9 ± 2 9.38
  1. *: estimated
  2. NB: non-binding
  3. Nd: not determined
  4. <Kdapp> = <koff>/<kon>
  5. The IC50 value corresponds to 50% inhibition of thrombin generation in the absence of PL for the different SVPLA2.