Figure 5

Binding assay of LIR2 with histamine. Binding assay was performed with 40 μl of concentrated 293T supernatant cell culture. The negative control used was a 10 time concentrated free-serum medium of untransfected cells. The supernatants were incubated with 100 nM ³H-histamine for 2 hours at 37°C. Protein precipitation with polyethylene glycol 8000 was used to separate bound from free histamine.