Figure 2

Circular dichroism spectroscopy. A. CD spectra of the CaM-peptide complex (red) and CaM (black). CaM was used at 0.050 mg/ml (in both samples) and the peptide at 0.030 mg/ml. B. Thermal stability curves of CaM (black) and the complex (red), monitored at 222 nm. Mean residue ellipticity as a function of temperature was measured during heating from +20 to +98°C (thick lines). Refolding of the samples during cooling from +98 to +20°C is indicated by the thin lines.